RNA Interference

Short interfering RNA (siRNA) is a double-stranded RNA molecule that is roughly 20 – 25 base pairs in length

siRNA interferes with the expression of genes by causing the mRNA transcripts to be broken prior to translation

  •  When the siRNA is unwound, one strand (the passenger strand) is degraded and one strand (the guide strand) remains
  • The guide strand will hybridise to its complementary mRNA sequence and recruit a protein complex called RISC
  • RISC (RNA induced silencing complex) will then destroy the targeted mRNA, preventing translation of the associated gene

RNA Interference with siRNA

RNA interference