Gene Knockouts


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•  Use of knockout technology in mice to determine gene function

A gene knockout is a genetic technique whereby a specific gene is rendered inoperative within an organism

  • Using the Cre-LoxP system, this gene knockout can be targeted to specific tissues within the organism (conditional knockout)

The Cre-LoxP system uses the enzyme Cre recombinase to remove genetic sequences located between two Lox sites

  • Using recombinant DNA technology, LoxP sequences are inserted on either side of a gene of interest in a test animal
  • The Cre gene is inserted next to a tissue-specific promoter in another test animal
  • When the two animals are bred, the resulting offspring will possess LoxP sequences and a tissue-specific Cre recombinase
  • The Cre recombinase will remove the gene located between the Lox sites to create a conditional knockout for further study

Creating a Conditional Knockout with the Cre-LoxP System

conditional knockout